Error bars in all plots are 95% confidence intervals (CI) calculated as percentiles of the bootstrap distribution obtained via case resampling. In Figure 4D, we used a generalized linear mixed model with Poisson output distribution and fixed effects contact type and cell type and random effect cell identity (R package lme4). The model yielded a significant intercept (z = 8.72, p<0.0001), a significant main effect of cell type (z = 4.11, p=4x10−4), a significant main effect of contact type (z = 2.66, p=0.008) and a significant interaction cell x contact type (z = −5.09, p<3.6x10−7).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.