2.3. Enrichment and Isolation of Atrazine Degrading Bacteria

Soil sample (10 g) was added to a 250 mL conical flask containing 100 mL AMSM (Atrazine purity: 50 mg L^–1), incubated aerobically at 30°C and shaken at 150 rpm for 7 days. Then, the enrichment culture (10 mL) was transferred to fresh AMSM containing Atrazine (100 mg L^–1). To repeat 6 times of this process to obtain the increased concentration of Atrazine (600 mg L^–1), the procedures of diluting the enrichment cultures and selecting of bacterial colonies were following those of Yang et al. [20]. A single bacterial strain FH-1 showing high biodegradation capacity was identified for further analysis.