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Micro-CT measurements of rat femurs
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After 12 weeks, all study rats were euthanized with sodium pentobarbital (150 mg/kg) (Sigma). Rat femurs were separated, and the surrounding soft tissue was removed. Femur samples were fixed in 4% paraformaldehyde for 72 hours. The 3-dimensional microarchitecture of the femurs was analyzed with a micro-CT system (μCT80; SCANCO Medical, AG, Switzerland) with energy settings of 55 kV and 145 μA, including bone mineral density (BMD), trabecular number (Tb.N), and trabecular spacing (Tb.Sp).
Copyright and License information: Med Sci Monit, ©2019
This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
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