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2.5. Cell Isolation and Culture

AS Andres Sanz-Garcia
CE Carmen Escobedo-Lucea
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Human adipose derived mesenchymal stem cells (hASCs) were isolated from lipoaspirates of young healthy donors undergoing aesthetic surgery (from 18 to 35 years-old), following written informed consent and Research Ethical Board approval by Clinica Isabel Moreno and Hospital General Foundation, Valencia, Spain. Donors were previously screened for Human Immunodeficiency Virus (HIV), hepatitis C and other infectious diseases. hASCs were expanded following the protocol described by Escobedo-Lucea et al. [32] and harvested with Tryple® (Invitrogen, Carlsbad, NM, USA) at 85% confluence.

Copyright and License information:  ©2018 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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