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6×105 low passage (<10) E14 cells were cultivated for 48 h in regular ES medium containing LIF. Four hours prior to cross-link, cells were treated with LIF or RA. Sequencing libraries were prepared, and the resulting DNA fragments were paired-end 50 bp sequenced on a Illumina HiSeq 2500 device. Raw sequencing reads were subsequently aligned to the genome assembly GRCm38 with STAR [70], and duplicates were removed using Picard tools [71].
Copyright and License information: The Author(s) ©2019
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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