2.4. Lentiviral-based shRNA transduction

MISSION shRNA lentiviral vectors were purchased from Sigma. The packaging of lentiviral particles was performed based on the manufacturer's protocol as supplied. Lentiviral transduction of 3T3-L1 cells was performed with shRNA targeting either all or long isoforms of Nrf1 (termed as A-Nrf1 and L-Nrf1, respectively) (see Fig. S2) and compared to non-target negative control (SHC002V) as described previously [7]. The non-target shRNA control vector activates RNA-induced silencing complex and RNA interference pathway, but does not silence any mouse genes. In the current study, we define the cells transduced with the non-target shRNA control vector as Scramble cells in contrast to knockdown (KD) cells.