Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Cells were detached from the culture dish with trypsin‐EDTA for 5 min and washed once with 1% BSA/PBS and incubated with either Alexa conjugated or unconjugated primary antibodies for 1 h at 4 °C. After labelling with secondary antibody, cells were analysed immediately by BD LSRII™ or BD FACSCalibur™ flow cytometers (BD Biosciences). Secondary antibody alone was used as the negative control for determining specificity of the signal.
Copyright and License information: The Author(s) ©2019
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
