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Identification using MicroSeq® microbial Identification System

VR Vibeke Børsholt Rudkjøbing
TT Trine Rolighed Thomsen
YX Yijuan Xu
RM Rachael Melton-Kreft
AA Azad Ahmed
SE Steffen Eickhardt
TB Thomas Bjarnsholt
SP Steen Seier Poulsen
PN Per Halkjær Nielsen
JE Joshua P. Earl
GE Garth D. Ehrlich
CM Claus Moser
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PCR was performed with primers that targeted the first 500 bases of the bacterial 16S rRNA gene or the D2LSU region of the fungal 28S rRNA gene. The resulting PCR products were sequenced using the MicroSeq® 500 kit (Applied Biosystems, Carlsbad, California) according to the manufacturer’s guidelines. The resulting DNA sequences were compared to the sequence library included in the MicroSeq® ID analysis software. In cases where sequencing resulted in mixed chromatograms due to 16S rRNA gene products from multiple species, these chromatograms were analyzed using RipSeq Mixed at www.ribseq.com.

Copyright and License information: The Author(s). ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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