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RNA was extracted from livers or cell samples using RNAse Mini Kit (Qiagen, Germantown, MD). A total of 5.0μg of RNA was reverse-transcribed into cDNA. Quantitative PCR was performed using Quant studio 3 (Applied Biosystems, Foster city, CA). The primers sequences are listed (Table S1). The expression of the target gene was normalized to the housekeeping HPRT or β2M.
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