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About 2000 cells of different genotypes were planted on 96-well plates and cultured at 37 °C for 24 h. After that, 20 mM EdU was added and continued to be cultured for 2 h. Cells were collected, fixed with 4% formaldehyde, stained with EdU Apollo@594 in vitro imaging kit (Ribo) according to the instructions. EdU incorporation rate was determined by leica inverted fluorescence microscopy.
Copyright and License information: The Author(s). ©2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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