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Enzymatic activity of invertase (EC: 3.2.1.26; encoded by SUC2) was assessed in yeast cell extracts according to previous protocols [61, 62] with the exception that the carcinogenic o-dianisidine was substituted with 4-aminophenazone/phenol [63]. A detailed description of the adapted protocol and calculation of the specific invertase activity can be found in Additional file 1. Assays were performed in biological triplicates.
Copyright and License information: The Author(s) ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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