BALF was collected to diagnose the cause of the ARDS within 24 h after intubation by instilling 100 to 150 mL of sterile isotonic saline, with gentle suctioning. BALF samples were immediately stored at − 80 °C. Blood was collected within 24 h after obtaining the BALF. Blood samples were centrifuged, and the serum was immediately stored at − 80 °C.
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