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Huh7 and HepG2 cells were transfected with miR-30b-3p mimics or anti-miR-30b-3p and their respective negative controls (miR-NC mimics and anti-miR-NC) or relative plasmids for 24 h. Then, cells were seeded in 96-well plates to obtain a cell density of 3 × 103 per well. Each group contained five duplicate wells. MTT (Sigma, USA) was then added to the 96-well plates to measure cell viability at 0, 24, 48 and 72 h, respectively. The absorbance (OD570) value was measured using a Microplate reader (Bio-Rad, USA).
Copyright and License information: ©2019 Gao, Zhou and Huang
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