4.7. DNMT Activity Assay

Cells were treated with 0.5% (v/v) DMSO, GSPs (20 μg/ML), Res (10 μM), and their combination in six-well plates for 48 h at 37 °C with 5% CO₂. The cells were then harvested, and nuclear extracts were prepared using EpiQuik Nuclear Extraction Kit (EpiGentek, Farmingdale, NY, USA), following the manufacturer’s protocol. DNMT activity assay was performed while using EpiQuik DNA Methyltransferase Activity/Inhibition Colorimetric Assay Kit (EpiGentek), following the manufacturer’s protocol.