Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Apoptosis was induced by PTX (0.25 and 2 μM) with or without EMSO (130 and 150 μM) co-treatment for 48 h each. Apoptosis detection was carried out with Annexin V-FITC and Propidium Iodide (PI) double staining assay kit (KeyGen Biotech, China), following the manufacturer’s instruction. Apoptotic cells were uncovered using flow cytometry (BD Bioscience, USA). Data acquisition and analysis were done with CellQuest V3.3 (BD Bioscience, USA).
Copyright and License information: The Author(s). ©2019
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
