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Flow cytometry analysis of the cell cycle
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Cells were seeded at a density of 1×106/mL and cultured in a 6-well plate. After adherence, the cells were synchronized for 12 hrs with serum-free media and treated with 0, 40, 80, or 160 μM eupatilin for 24 hrs. Cells were harvested and fixed in 75% cold ethanol for 12 hrs and washed twice with PBS. Cells were incubated with 100 mg/mL RNase A and 50 mg/mL propidium iodide at 37°C for 30 mins. The cell cycle was analyzed by flow cytometry.
Copyright and License information: ©2019 Fei et al.
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