Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
The rat glial C6 cells (1 × 105 C6 cells/ml) were cultured in a 6-well plate for 24 h. After removing the culture medium, the cells were washed with PBS. C6 cells were cultured in DMEM without FBS (500 μl) and incubated with Acanthamoeba EVs (100 μg) in PBS. The total volume of culture medium was adjusted to 1 ml. DMEM without FBS (500 μl) combined with PBS (500 μl) served as a negative control. After 24 h of co-incubation, the cells were washed, paraformaldehyde-fixed, and then stained with Giemsa stain (Merck, Darmstadt, Germany) [36].
Copyright and License information: The Author(s) ©2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
