Formation of RADA16-I–MA in situ hydrogel and rheological analysis of in situ hydrogel

The in situ hydrogel was prepared by quickly adding 100 μL RADA16-I–MA colloidal suspension to PBS (pH 7.4) and allowed to stand for 10 min to observe the gel state.

The dynamic rheological characterization of the RADA16–MA suspension and hydrogel was performed on a DHR-1 rheometer (TA Instruments, New Castle, DE, USA).RADA16-I–MA suspension was transferred to the rheometer plate, and the parallel plate was then lowered to a gap of 250 µm. For the RADA16-I–MA hydrogel, RADA16-I–MA suspensions were mixed with PBS quickly at a volume ratio of 1:1 and then placed on a rheometer plate. After equilibration for 5 min, the parallel plate was then lowered to a gap of 250 µm. Subsequently, G’ and G’’ were measured following the procedure already described.