2.3. Immunohistochemistry (IHC)

LQ Lu Qiao
NX Ning Xie
YB Yuru Bai
YL Yan Li
YS Yongquan Shi
JW Jinhai Wang
NL Na Liu
ask Ask a question
Favorite

The avidin-biotin complex immunoperoxidase method was reported in a previous study [19]. The section was incubated with monoclonal mouse anti-HNRNPL (sc-32317, Santa, USA) at 1:200 dilution. According to staining intensity in the majority of specimens, immunoreactivity was scored as absent (−), weak (+), moderate (++), or strong (+++) [19].

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A