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Limiting dilution analysis (LDA)

JW Jinhua Wang
YX Yajing Xing
YW Yingying Wang
YH Yundong He
LW Liting Wang
SP Shihong Peng
LY Lianfang Yang
JX Jiuqing Xie
XL Xiaotao Li
WQ Wenwei Qiu
ZY Zhengfang Yi
ML Mingyao Liu
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For in vitro LDAs, QW24 pretreated HCT116 cells were dissociated into single cells and seeded in 96-well plates at indicated cell doses. For each cell dose, at least 8 wells were seeded with cells, and for the lower cell doses (10 cells or single cell per well), 12 wells were plated. After 1 week incubation, wells containing spheres were scored, and the number of positive wells was used to calculate the frequency of sphere-forming units using the ELDA software (http://bioinf.wehi.edu.au/software/elda/index.html) provided by the Walter and Eliza Hall Institute [33].

Copyright and License information: The Author(s). ©2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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