Effect of antibodies to actin tail formation

RAW264.7 cells were cultivated on sterilized glass slides and infected with YFP-expressing L. monocytogenes. After incubation for 30 min, the extracellular bacteria were eliminated by gentamicin. At 6 h after infection, the cells were fixed with 4% paraformaldehyde for 15 min at room temperature and washed with PBS. The cells were then permeabilized with 0.5% Triton X-100 in PBS. Actin filaments were stained with 100 nM rhodamine-conjugated phalloidin (Cytoskeleton Inc., Denver, CO) and counterstained with DAPI. Fluorescent signals were observed under fluorescence microscope.