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Shake flask cultivations

AE Anna Maria Erian
MG Martin Gibisch
SP Stefan Pflügl
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Shake flask cultivation were carried out with a working volume of 100 ml in shake flasks with a total volume of 500 ml. An initial OD600 of 0.5 was used for all shake flask cultivations. Shake flasks were incubated at 37 °C and 200 rpm. Samples of E. coli cultivations were taken immediately after inoculation, after 24 h and after 48 h. In case of pulsed fed-batches, samples were taken before and after addition of molasses-medium solution to the culture.

Copyright and License information: The Author(s) ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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