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Degradation of chitosan and α- or β-crystalline chitin substrates by pepsin preparations

ET Eri Tabata
SW Satoshi Wakita
AK Akinori Kashimura
YS Yasusato Sugahara
VM Vaclav Matoska
PB Peter O. Bauer
FO Fumitaka Oyama
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Chitosan [degree of deacetylation (DD) 80%, Chitosan 100] and α-chitin from shrimp shell chitin were purchased from FUJIFILM-Wako Pure Chem. Co. and Sigma-Aldrich, respectively. β-chitin from squid pens was a generous gift from Katakura & Co-op Agri Corporation (Tokyo, Japan). DD was determined by elemental analysis as described previously20. Chitosan, α- or β-crystalline chitin substrate (1 mg/mL) was incubated in a volume of 50 µL containing pepsin preparation (P7012, Sigma-Aldrich) (0.5 mg/mL) or Pep A (0.5 mg/mL) in McIlvaine’s buffer (pH 4.0) at 37 °C for 16 hours. Generated chitin fragments were analyzed by FACE28,29.

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