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The paraffin-embedded kidney specimens were sectioned at 3 μm thickness, deparaffinized, stained with PAS reagent to identify kidney structure and hematoxylin for counterstaining. The sections were placed on glass slides, deparaffinized, stained with PAS and dehydrated with absolute alcohol. The stained tissue sections were observed using an optical microscope Axioimager system and images were obtained for each section.
Copyright and License information: ©2018 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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