Assessment of gastric acid secretion

Determination of gastric acid was carried out based on the methods used in previous studies.14,33,34 After the WIR treatment for 12 hrs, all rats from five groups were rounded up and anesthetized with isoflurane, and then the rat’s abdomen was opened by midline laparotomy and the stomach was fully exposed, the pylorus was then ligated and the abdominal incision was temporarily closed. Two hours later, the incision was opened again, the lower end of the esophagus was ligated and the entire stomach was then separated. The gastric content was emptied, transferred into graduated centrifuge tubes and centrifuged for 10 mins (4°C, 3000× g). The pH of the supernatant obtained after centrifugation was measured and recorded with a pH meter (METTLER TOLEDO, Zurich, Switzerland)