Measurement of mitochondrial function

Shao-Hua Su; Yi-Fang Wu; Da-Peng Wang; Jian Hai

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Measurement of mitochondrial function

SS Shao-Hua Su

YW Yi-Fang Wu

DW Da-Peng Wang

JH Jian Hai

This method is extracted from research article: Cell Death Dis, Jun 2018

Inhibition of excessive autophagy and mitophagy mediates neuroprotective effects of URB597 against chronic cerebral hypoperfusion

DOI: 10.1038/s41419-018-0755-y

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The mitochondrial function was measured using a JC-1 staining kit (Genmed Scientifics Inc., Minneapolis, MN, USA). Briefly, 10 μl of high-purity mitochondria (containing 5 μg mitochondrial proteins) were incubated in JC-1 staining reagent (100 μl) for 10 min. Images were captured using a microscope (Olympus IX71; Olympus, Tokyo, Japan) at wavelengths of 594 and 488 nm. Healthy mitochondria, which have a high potential, showed a high intensity of red fluorescence at 594 nm and a low intensity of green fluorescence at 488 nm; damaged mitochondria showed a low intensity of red fluorescence at 594 nm and a high intensity of green fluorescence at 488 nm. The ratio of red/green intensity was measured to evaluate the function of isolated mitochondria from each group.

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