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Kidney histopathological examination

YF Yuyan Fan
HF Hongyu Fan
BZ Bin Zhu
YZ Yilun Zhou
QL Qingshan Liu
PL Ping Li
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To determine the histopathological changes in kidney, the kidneys from rats were staining by ematoxylin-Eosin (HE). The kidney of the rat was fixed in 10% formalin and embedded in paraffin. After deparaffinization and rehydration, 5 µm thickness kidney sections were measured by Hematoxylin-Eosin Staining (WLA051a, Wanleibio, China) as described by the manufacturer. Finally, the sections were observed and photographed by an optical microscope (DP73, OLUMPUS, Japan).

The kidney tissues were dehydrated, embedded in paraffin, sectioned at 5 μm thickness, and stained by Periodic Acid-Schiff (PAS) (BA-4114, Baso, China) stain. Each sample slice was observed under the microscope (DP73, OLUMPUS, Japan) at a magnification of 200 × .

Copyright and License information: The Author(s). ©2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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