siRNA Transfection

Cells of the cervical cancer cell line SiHa were transfected with 25 nM non-targeting control siRNA or synthetic siRNAs (Wuhan GeneCreate Biological Engineering) against lncRNA799 (si-lnc799#1: 5′-GGAUGGCUUUAAUUCUCUUTT-3′ and 5′-AAGAGAAUUAAAGCCAUCCTT-3′; si-lnc799#2: 5′-CCACUUCUCUUGCCCAUAUTT-3′ and 5′-AUAUGGGCAAGAGAAGUGGTT-3′) for 48 hr using the Lipofectamine transfection reagent (Invitrogen). The cells were then collected, and lncRNA799 knockdown was confirmed by qRT-PCR. siRNAs against TBL1XR1 were generated as previously described.7