Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

TOP/FOP flash reporter assay

YS Yang Song
GL Gao Liu
SL Shuang Liu
RC Rong Chen
NW Na Wang
ZL Zhaoyu Liu
XZ Xiao Zhang
ZX Zheng Xiao
LL Lin Liu
ask Ask a question
Favorite

A TOP/FOP flash assay was used for Wnt signaling pathway analysis. Briefly, the TCF/LEF-responsive luciferase construct was made under the control of minimal TK promoter and tandem repeats of the TCF/LEF transcriptional response element. GC cells were seeded in a 24-well plate and co-transfected with the pRL-TK plasmid (10 ng/well) and either TOP flash plasmid or FOP flash plasmid (200 ng/well), and then treated by H. pylori and inhibitors as indicated. At 48 hrs later, the cells were analyzed using the Dual-Luciferase Reporter Assay System (Promega, E1910). The ratio of TOP Flash activity and FOP Flash activity represented the results of the activity.

Copyright and License information:  ©2019 Song et al.
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A