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Prediction of TF and miRNA

MI Mir Asif Iquebal
PS Pradeep Sharma
RJ Rahul Singh Jasrotia
SJ Sarika Jaiswal
AK Amandeep Kaur
MS Monika Saroha
UA U. B. Angadi
SS Sonia Sheoran
RS Rajender Singh
GS G. P. Singh
AR Anil Rai
RT Ratan Tiwari
DK Dinesh Kumar
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Blastx search (e-value 1e-05) was used for identification of transcriptional factors (TFs) against PlantTFDB (Plant Transcriptional Factors Database) version 4.063 for each of the four sets, namely, TC:TD, SD:TD, SC:SD and SC:TC. Further, 119 mature miRNA of Triticum aestivum were used for predicting their targets in the differentially expressed genes using psRNATarget webserver64,65 (http://www.mirbase.org/). For validation of predicted miRNA from each set, their BLAST analysis with strict stringency criteria such as 100% identity and zero mismatch against publically available wheat root drought specific small RNA library (NCBI/SRA SRR1055298) was done.

Copyright and License information: The Author(s) ©2019
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