DNA extraction

DB Diego Bogarín
OP Oscar A. Pérez-Escobar
AK Adam P. Karremans
MF Melania Fernández
JK Jaco Kruizinga
FP Franco Pupulin
ES Erik Smets
BG Barbara Gravendeel
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We extracted total genomic DNA from about 50–100 mg of silica gel dried leaf/flower tissue. Each sample was placed in 2 ml Eppendorf® tube with three glass beads (7 mm) and sterile sand. The tubes were frozen in liquid nitrogen for about 1–2 minutes and powdered in a Retsch MM 300 shaker for 3 minutes. We followed the 2 × CTAB (Hexadecyltrimethylammonium bromide) protocol for isolating DNA44. DNA was quantified with a Qubit 3.0 Fluorometer (TermoFischer Scientific®).

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