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Genes in which the expression varied between different samples were selected with the FindVariableGenes function in the SEURAT package [47]. Genes were first divided into 20 bins based on their average expression levels across all samples. The log-transformed values of the variance divided by the mean, as a measure of dispersion, were then calculated in each bin and z-scored. A set of variable genes were then obtained by setting the threshold of the dispersion value > 0.8 and the average log2-transformed FPKM value > 3 (Additional file 3: Figure S2).
Copyright and License information: The Author(s). ©2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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