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Preparation of nile red

MB Marie N. Bongiovanni
JG Julien Godet
MH Mathew H. Horrocks
LT Laura Tosatto
AC Alexander R. Carr
DW David C. Wirthensohn
RR Rohan T. Ranasinghe
JL Ji-Eun Lee
AP Aleks Ponjavic
JF Joelle V. Fritz
CD Christopher M. Dobson
DK David Klenerman
SL Steven F. Lee
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Phosphate-buffered saline (PBS, 00-3002, ThermoFisher scientific) was freshly prepared using high-purity Milli-Q water of resistivity 18 MΩ cm and filtered (0.02 μm cut-off, Whatman) and used without further pH adjustment. Nile red (NR) stock solutions were prepared by dissolving NR into dimethyl sulfoxide (DMSO, D2650 >99.7% purity) and then diluted into pre-filtered PBS buffer and used at a final concentration of 0.05 μM unless otherwise specified.

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