Determination of the minimum fungicidal concentration

The minimum fungicidal concentration (MFC) was determined by inoculating the contents from the MIC plates onto SDA plates, and the results were observed after incubation at 37°C at various durations depending on the fungi. The presence of the fungal colonies on the agar plates was an indication that the plant extract only inhibited the growth of the fungi without killing them, and the absence indicated that the plant extract was able to kill the fungal organisms.[36] The smallest concentration of the plant extract that was able to kill the microorganisms was considered MFC.