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Total cell RNA was extracted by Trizol and reversed to cDNA for PCR using reverse transcription reagents (TIANGEN, Beijing, China). Then CBX8 and GAPDH were amplified by PCR and detected on a 1.5% agarose gel. PCR primers for CBX8 and GAPDH mRNA detection were as follows: CBX8 (F: 5′-TAAGGAAAGTAACACGGACCAA-3′, R: 5′-GAAATAAAAATCACTATGCCAA-3′), GAPDH (F: 5′-CCGCATCTTCTTTTGCGTCG-3′, R: 5′-AGTTAAAAGCAGCCCTGGTGA-3′).
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