Correlation analysis of the antibody binding data was performed by using WGCNA package31 in Microsoft R open 3.5.1 (https://mran.microsoft.com/open). Pearson’s correlation coefficient between Ab-peptide binding intensities was calculated by using “cor” function in Microsoft R open 3.5.1. Statistical significance of the correlation was calculated by using Fisher’s exact test (two-sided) which is integrated in “corPvalueFisher” function in WGCNA package. Data was visualised by using “labeledHeatmap” function in WGCNA package in Microsoft R open 3.5.1.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.