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NCI‐H660 NEPC cells were grown in RPMI‐1640 (Hyclone) containing 5% fetal bovine serum (GIBCO), 1% Insulin‐Transferrin‐Selenium (Thermo Fisher), 10 nmol/L hydrocortisone (Sigma), 10 nmol/L beta‐estradiol (Sigma), and 1% Matrigel (Corning). Cells were dissociated for passaging and various assays using Accutase (Stemcell Technologies).
Copyright and License information: ©2018 The Authors. published by John Wiley & Sons Ltd.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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