Materials

All culture materials were purchased from Gibco (Grand Island, NY). LY294002 (PI3K/Akt inhibitor) and rapamycin (p70S6K inhibitor) were purchased from Calbiochem (La Jolla, CA). Mouse monoclonal antibodies (mAB) against SREBP-1c, NLRP3, IL-1β, pro-IL-1β, caspase-1, pro-caspase-1, phospho-Akt, and Akt were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Rabbit polyclonal antibodies against phospho-p70S6K, p70S6K, phospho-JAK2, and JAK2 were purchased from Cell Signaling Technology (Beverly, MA). SREBP-1c, NLRP3, JAK2 siRNA and control siRNA were purchased from the National RNAi Core Facility in Academic Sinica, Taipei, Taiwan. The integrin β1-siRNA were purchased from Invitrogen (Carlsbad, CA). P. gingivalis lipopolysaccharide (Pg-LPS) was purchased from InvivoGen (San Diego, CA). Other chemicals of reagent grade were obtained from Sigma (St. Louis, MO).