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Samples and reference isolates

CV Claudia A. Vera-Arias
LC L. Enrique Castro
JG Javier Gómez-Obando
FS Fabián E. Sáenz
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Samples used in this study were collected from patients between January 2013 and March 2016 by the personnel of the Ministry of Health of Ecuador. A total of 41 blood samples (4 samples in 2013; 15 in 2014; 14 in 2015 and 8 in 2016) were collected in San Lorenzo county from patients who initially reported to be microscopically positive for P. falciparum infection, and from whom informed consent was obtained. The blood samples were collected by finger prick or by drawing peripheral whole blood and spotted on 3MM Whatman filter paper. Thirty-two samples from an outbreak in Esmeraldas city, between November 2012 and November 2013 and reported in Saenz et al. [7] were also included in the study. Fifty-six percent of the samples (23/41) were collected by finger prick or by drawing peripheral whole blood and 44% of samples (18/41) were collected only by rapid diagnostic tests (RDT) with a positive result.

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