Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Intrathecal injection and drugs

YH Yang Huang
LW Li-Li Wen
JX Jing-Dun Xie
HO Han-Dong Ouyang
DC Dong-Tai Chen
WZ Wei-An Zeng
request Request a Protocol
ask Ask a question
Favorite

Under isoflurane anesthesia, apolyethylene (PE-10) catheter was inserted through the foramen magnum and advanced 8 cm caudally to the intumescentia lumbalis, as previously described.24 Rats that appeared to have hind limb paralysis or paresis after surgery were excluded from this study. Intrathecal injections were performed four to five days after catheter implantation. We administered KB-R7943 (Tocris, UK), which was dissolved in 1% dimethyl sulfoxide (DMSO). The KB-R7943 was intrathecally injected in a 10 µL volume, followed by a 10 µL saline flush to assure drug delivery to the subarachnoid space. The control group received 1% DMSO.

Copyright and License information: The Author(s) ©2019
Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A