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Boyden chamber assay

KC Keri Callegari
SM Shinji Maegawa
JB Javiera Bravo-Alegria
VG Vidya Gopalakrishnan
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24-well transwell Matrigel migration assay plates and control inserts were purchased from Corning (Catalog #354480). Briefly, approximately 2.5 × 104 cells were seeded in 8 μM pore-size transwell chambers. The bottom chamber was filled with 20% FBS to act as a chemoattractant. Cells were allowed to incubate for 22 h and were then fixed, stained with Hemistat triple pack and imaged under a microscope. Cell nuclei were counted from 4× images taken of the membrane insert. CellCounter software was used to automate the counting process. Cells were plated in triplicate and averaged.

Copyright and License information: The Author(s). ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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