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Wound-healing assay was performed to detect to changes in cell migration. Cells were seeded in 6-well plates, and grew to confluent, and then a scratch wound was made with a 200-μl pipette tip in a straight line. After rinsing with serum free RPMI-1640 medium (SFM), cells were incubated in a condition of 37 °C under 5% CO2 for 24 hrs. Cell images were captured under a microscope at 0 hr and 24 hrs post-wounding.
Copyright and License information: ©2019 Man et al.
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