Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
Wound-healing assay was performed to detect to changes in cell migration. Cells were seeded in 6-well plates, and grew to confluent, and then a scratch wound was made with a 200-μl pipette tip in a straight line. After rinsing with serum free RPMI-1640 medium (SFM), cells were incubated in a condition of 37 °C under 5% CO2 for 24 hrs. Cell images were captured under a microscope at 0 hr and 24 hrs post-wounding.
Copyright and License information: ©2019 Man et al.
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
