RNA-seq

NL Ning Li
LY Lu Yang
XQ Xue-Kang Qi
YL Yu-Xin Lin
XX Xinhua Xie
GH Gui-Ping He
QF Qi-Sheng Feng
LL Ling-Rui Liu
XX Xiaoming Xie
YZ Yi-Xin Zeng
LF Lin Feng
ask Ask a question
Favorite

CNE2-EBV−/+ and TWO3-EBV−/+ cells were treated with 0.1% DMSO or 1 μM JQ1 for 3 h, and mRNA was extracted using an AllPure Cell Kit (Magen, Guangzhou, China) according to the manufacturer’s instructions and submitted to Beijing Novogene Bioinformatics Technology Company Limited for RNA-seq analysis. Analysis of RNA-seq data and gene set enrichment analysis (GSEA) were performed by Guangzhou Genedenovo Bio-Technology Company Limited. A detailed description of the GSEA methodology is provided at http://www.broadinstitute.org/gsea/doc/GSEAUserGuideFrame.html.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A