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Microglial BV-2 cells were plated in 12-well plates (1 × 105 cells/well) and were transiently transfected with siRNA for 30 min, using a mixture of CHI3L1 siRNA (20 nM) and the RNAiMAX reagent in OPTI-MEM, according to the manufacturer’s specification (Invitrogen). The transfected cells were treated with K284-6111 (0.5 μM) for 24 h and then used for detecting protein expression.
Copyright and License information: The Author(s). ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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