Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Plasmids, shRNA, sulfatide, GalCer, and antibodies

QC Qianhua Cao
XC Xingyu Chen
XW Xuebiao Wu
RL Ruocen Liao
PH Panpan Huang
YT Yanjia Tan
LW Li Wang
GR Guoping Ren
JH Jian Huang
CD Chenfang Dong
request Request a Protocol
ask Ask a question
Favorite

UTG8 shRNA was purchased from MISSION shRNA at Sigma-Aldrich. Human UGT8 and Sox10 were amplified from a MDA-MB231 cDNA library and subcloned into pLVX-Puro.

Sulfatide and GalCer were purchased from Avanti Polar Lipids and Abcam, respectively. Antibody against UGT8 was from Protein Tech Group. Antibodies against Sox10, Smad4, Smad5, Integrin αV, and Integrin αVβ5 were from Abcam. Antibodies against RelA and Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) were from Santa Cruz and Cell Signaling Technology, respectively. Antibodies for galactocerebroside, sulfatide, and integrin αVβ3 were purchased from Millipore Sigma. Antibodies for ID4 and β-actin were from BioCheck and Sigma-Aldrich, respectively.

Copyright and License information:  ©2018 Cao et al.
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A