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Cell viability was assessed by MTT assay. Cells were seeded in 96-well plates and treated with celecoxib and/or imatinib for 24, 48 or 72 h. Then MTT was added and incubated for 4 h, followed by centrifugation at 1500 rpm for 5 min. Supernatants were removed and the remaining MTT dye was solubilized with 200 μl DMSO. The optical density was measured at 490 nm using a multi-well plate reader (Micro-plate Reader; Bio-Rad, Hercules, CA).
Copyright and License information: The Author(s) ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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