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An in house R-tool was applied to perform paired Limma test (moderated t-test) with correction for multiple testing to allow for the detection of significant protein and PTM level changes in the proteomic dataset (Storey, 2002; Schwämmle et al., 2013). Analyses of functional assays were performed in Graphpad Prism version 5.0 (GraphPad Software, United States) using two-tailed Student’s T-tests or one-way ANOVA with Tukey’s post hoc test where appropriate. Results are expressed as mean ± SEM, and P-values < 0.05 were considered statistically significant.
Copyright and License information: ©2019 Bogetofte, Jensen, Ryding, Schmidt, Okarmus, Ritter, Worm, Hohnholt, Azevedo, Roybon, Bak, Waagepetersen, Ryan, Wade-Martins, Larsen and Meyer.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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