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Green fluorescent protein (GFP) mice

MH Masahiro Hatakeyama
MK Masato Kanazawa
IN Itaru Ninomiya
KO Kaoru Omae
YK Yasuko Kimura
TT Tetsuya Takahashi
OO Osamu Onodera
MF Masanori Fukushima
TS Takayoshi Shimohata
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To determine whether transplanted PBMCs can translocate from the blood into the brain parenchyma to exert their beneficial effects after intra-arterial administration, PBMCs from GFP mice were used31. GFP transgenic mice were produced by breeding heterozygous pairs in the Genome Information Research Centre, Osaka University, Japan and maintained in the Department of Comparative and Experimental Medicine, Brain Research Institute, Niigata University. Following the preconditioning of primary PBMCs from GFP mice by OGD, these cells were administered intra-arterially at 7 days after cerebral ischemia. Finally, a confocal microscopic examination was conducted at 3 and 21 days after administration in triplicates9.

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