Animal experiments

Four-week-old, male C57BL/6 J mice were obtained from the Jackson Laboratory (Bar Harbor, ME). All animal experiments were reviewed and approved by the University of Utah Institutional Animal Care and Use Committee (Protocols #15-08004 and #18-06006) and conducted in accordance with the guidelines prepared by the Committee on Care and Use of Laboratory Animals, Institute of Laboratory Animals Resources, National Research Council. Animals were maintained at a maximum of five mice per cage on a 12 h light/12 h dark cycle at 22 °C. Food and water were available ad libitum. Mice were maintained on Teklad sterilizable rodent diet (autoclaved diet) (cat. #8656; Harlan Laboratories, Indianapolis, IN). All efforts were made to minimize suffering. Mice were euthanized through an overdose of isoflurane.