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Cell migration behavior was evaluated using the wound healing assay as previously described.24 Briefly, monolayers were wounded with a plastic pipette tip, the dead cells were removed by rinsing, and serum-free media was added. At the designated times (0 h, 12 h, and 24 h), images of three randomly selected fields at the lesion border were acquired using an Olympus IX-71 inverted microscope. The wound healing rate was calculated based on the captured images.
Copyright and License information: ©2019 Wang et al.
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